Immediate induction of APETALA1-like gene expression following a single short-day in Pharbitis nil
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چکیده
منابع مشابه
Changes in catecholamine levels in short day-induced cotyledons of Pharbitis nil.
We investigated the effects of catecholamine on flower-induction in P. nil (cv. Violet). GC-SIM analysis identified dopamine for the first time in P. nil seedlings. Dopamine levels in the cotyledons did not show a significant change during the inducing dark treatment. The dopamine content of cotyledons exposed to various durations of darkness were 0.1-0.2 nmol/g fresh weight. The same content w...
متن کاملPhytochrome regulation of phytochrome A mRNA levels in the model short-day-plant Pharbitis nil.
The exposure of dark-grown Pharbitis nil seedlings to continuous R induces a rapid decrease in PHYA mRNA abundance with a half-life of about 2 h. A 5 min R pulse also induces this decline, and the effect is partially reversible by subsequent FR irradiation, confirming that the regulation of expression is mediated via the Pfr form of a phytochrome. When de-etiolated seedlings are returned to dar...
متن کاملConstitutive expression of the GIGANTEA ortholog affects circadian rhythms and suppresses one-shot induction of flowering in Pharbitis nil, a typical short-day plant.
GIGANTEA (GI) is a key regulator of flowering time, which is closely related to the circadian clock function in Arabidopsis. Mutations in the GI gene cause photoperiod-insensitive flowering and altered circadian rhythms. We isolated the GI ortholog PnGI from Pharbitis (Ipomoea) nil, an absolute short-day (SD) plant. PnGI mRNA expression showed diurnal rhythms that peaked at dusk under SD and lo...
متن کاملTiming of growth inhibition following shoot inversion in Pharbitis nil.
Shoot inversion in Pharbitis nil results in the enhancement of ethylene production and in the inhibition of elongation in the growth zone of the inverted shoot. The initial increase in ethylene production previously was detected within 2 to 2.75 hours after inversion. In the present study, the initial inhibition of shoot elongation was detected within 1.5 to 4 hours with a weighted mean of 2....
متن کاملStructure and Light-Induced Expression of a Small Heat-Shock Protein Gene of Pharbitis nil.
To isolate genes that are regulated by a photoperiod that promotes flowering in Pharbitis nil, a cDNA library representing mRNA of induced cotyledons was screened by differential hybridization. The DNA sequence of one cDNA clone isolated by this approach, clone 12L, showed homology to plant small heat-shock protein (hsp) genes. P. nil genomic clones hybridizing to clone 12L were isolated, and t...
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ژورنال
عنوان ژورنال: Plant Biotechnology
سال: 2008
ISSN: 1347-6114,1342-4580
DOI: 10.5511/plantbiotechnology.25.565